SRP0273
PDE8A1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonym(s):
PDE8, PDE8A, Phosphodiesterase 8A1
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10 μG
€647.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥437 pmol/min-μg
mol wt
119 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE8A(5151)
General description
PDE8A (phosphodiesterase 8A) is one of the two subfamilies of PDE8 protein family, the other being PDE8B. PDE8 shows wide range of tissue expression, and is especially abundant in testis.[1] PDE8A contains a Per, ARNT and Sim (PAS) signaling domain in its N-terminal. PDE8s contain receiver (REC) domain, N-glycosylation, N-myristoylation, amidation consensus sites, and potential kinase substrate sites for PKC (protein kinase), PKA and casein kinase.[2]
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
PDE8 (phosphodiesterase 8) are cAMP-specific enzymes, and they play a role in various processes such as in T-cell activation regulation, chemotaxis of activated lymphocytes, control of testosterone synthesis in Leydig cells, initiation of glucose-induced biphasic insulin response, steroidogenesis, and excitation–contraction coupling.[1][2] rs2304418 variant in this gene is linked with lower mRNA expression of PDE8A, which in turn is linked with reduced HIV-1 (human immunodeficiency virus) replication. The expression of this protein is significantly high in macrophages, and is controlled by miR-145-5p. PDE8A promotes HIV-1 replication in macrophages and this is controlled at the level of reverse transcription.[3][4]
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Refolding and kinetic characterization of the phosphodiesterase-8A catalytic domain.
Yan Z et al
Protein Expression and Purification, 64(1), 82-88 (2009)
Cyclic AMP-specific phosphodiesterase, PDE8A1, is activated by protein kinase A-mediated phosphorylation.
Brown KM et al
Febs Letters, 586(11), 1631-1637 (2012)
Polymorphism in HIV-1 dependency factor PDE8A affects mRNA level and HIV-1 replication in primary macrophages.
Bol SM et al
Virology, 420(1), 32-42 (2011)
Thijs Booiman et al.
PloS one, 9(10), e109673-e109673 (2014-10-09)
HIV-1 infected macrophages play a key role in HIV-1 infection. Even during anti-retroviral treatment, macrophages keep producing virus due to suboptimal tissue penetration and reduced efficacy of antiretrovirals. It is therefore of major importance to understand which host factors are
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