SHC015V
MISSION® pLKO.1-puro-UbC-TagFP635™ Positive Control Transduction Particles
Contains a gene encoding TagFP635, under the control of the UbC promoter
Synonym(s):
MISSION®, MISSION® Control Transduction Particles
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About This Item
UNSPSC Code:
12352200
product line
MISSION®
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
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General description
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TagFP635 Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TagFP635 Control Transduction Particles are produced from the sequence-verified 9226 base pair lentivirus plasmid, pLKO.1-puro-UbC-TagFP635 (SHC015).
This construct aids in interpretation of experimental design and results by providing a far-red fluorescent protein marker for monitoring success in transduction of your cells of interest.
This construct aids in interpretation of experimental design and results by providing a far-red fluorescent protein marker for monitoring success in transduction of your cells of interest.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagFP635 is a trademark of Evrogen Co.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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