R9404

Anti-RhoA antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-p21 Rho
• MDL number: MFCD09265373
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~21 kDa
• species reactivity: monkey, mouse, human
• concentration: ~2 mg/mL
• technique(s): western blot: 1-2 μg/mL using HEK293 cells expressing human RhoA/ Hela cell extract
• UniProt accession no.: P61586
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RHOA(387)

Description

General description

RhoA (ras homolog family member A) belongs to the Rho GTPase family of proteins. It is located on chromosome 3p21.31.

Specificity

Anti-RhoA recognizes human RhoA.

Immunogen

synthetic peptide corresponding to amino acids 177-189 located near the C-terminus of human RhoA.

Biochem/physiol Actions

In multiciliated epithelial cells, RhoA (ras homolog family member A) controls the actin network dynamics. It also modulates membrane protrusion and contractility in the cell body. It is one of the essential factor for Ras transformation. It is associated with cancer metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in "frost-free" freezer, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. For continuous use, store at 2-8°C for up to one month.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves