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Millipore

ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

technique(s)

immunodepletion: suitable

storage temp.

2-8°C

General description

ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.

Application

ProteoPrep® Immunoaffinity Albumin & amp; IgG Depletion Kit has been used in:
  • umbilical cord serum proteomics
  • human plasma (HP) proteomics
  • proteomic profile-based screening of potential protein biomarkers in the urine of patients with nephrotic syndrome

Features and Benefits

  • High Capacity removes more interfering abundant proteins
  • High Specifity ensures you see an accurate and more complete proteome
  • Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

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Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n

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