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PROTDT

Millipore

ProteoPrep® Detergent Sample Kit

Synonym(s):

protein solubilization detergent

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1 KIT
€514.00

€514.00

Estimated to ship onApril 16, 2025

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1 KIT
€514.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

€514.00

Estimated to ship onApril 16, 2025

shelf life

2 yr

Quality Level

200

storage temp.

15-25°C

General description

The ProteoPrep® Detergent Sample Kit provides ten detergents, non-ionic and zwitterionic, for protein solublization. The variety of innovative detergents enables optimization of extraction methods, which may vary with the protein of interest and cell type. Zwitterionic detergents uniquely offer some intermediate class properties that are superior to other detergent types. Each detergent is supplied in a convenient package size, ideal for method development and experimentation.

Application

ProteoPrep® Detergent Sample Kit has been used as a component of sample buffer for hepatoblastoma (HB) HepG2 cells.[1]

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description
  • Brij® C10 1 g

Kit Components Also Available Separately

Product No.
Description
SDS
  • A1346ASB-14 1 gSDS
  • C0856C7BzO 1 gSDS
  • P2393Polyoxyethylene (10) tridecyl ether, mixture of C11 to C14 iso-alkyl ethers with C13 iso-alkyl predominating 1 gSDS
  • C9426CHAPS hydrate, BioReagent, suitable for electrophoresis, ≥98% (HPLC) 1 gSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 1 gSDS
  • O8001Octyl β-D-glucopyranoside, ≥98% (GC) 1 gSDS
  • O6004Octyl β-D-1-thioglucopyranoside, ≥98.0% (GC) 1 gSDS
  • D42663-(Decyldimethylammonio)­propane­sulfonate inner salt, zwitterionic detergent 1 gSDS
  • T8532Triton™ X-100, suitable for electrophoresis 1 mLSDS
See All (9)

Signal Word

Danger

Hazard Statements

H302,H315,H318,H360D,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCD5127
SLBX7013
SLBT0335
SLBT0334
SLBQ0288V

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NOX1 supports the metabolic remodeling of HepG2 cells
Bertram K, et al.
Testing, 10(3), e0122002-e0122002 (2015)
Katharina Bertram et al.
PloS one, 10(3), e0122002-e0122002 (2015-03-26)
NADPH oxidases are important sources of reactive oxygen species (ROS) which act as signaling molecules in the regulation of protein expression, cell proliferation, differentiation, migration and cell death. The NOX1 subunit is over-expressed in several cancers and NOX1 derived ROS

Questions

  1. How much of each surfactant and in what state (solid or solution, if solution what concentration) are the surfactants provided in this kit?

    1 answer

    1. As listed in the product data-sheet:

      Components
      CHAPS (Catalog Number C9426) 1 g - powder form
      C7BzO (Catalog Number C0856) 1 g - powder form
      ASB-14 (Catalog Number A1346) 1 g - powder form
      3-(Decyldimethylammonio) propanesulfonate inner salt (Catalog Number D4266) 1 g - powder form
      n-Dodecyl b-D-maltoside (Catalog Number D4641) 1 g - powder form
      Octyl b-D-glucopyranoside (Catalog Number O8001) 1 g - powder form
      Octyl b-D-1-thioglucopyranoside (Catalog Number O6004) 1 g - powder form
      Polyoxyethylene 10 tridecyl ether mixture of C11 to C14 iso-alkyl ethers with C13 iso-alkyl predominating (Catalog Number P2393) 1 g - liquid form, neat
      Brij C10 (Catalog Number P5759) 1 g - 'solid' form, melting point 32 - 34 °C, density in liquid form 0.977 g/mL
      TRITON X-100 (Catalog Number T8532) 1 ml - liquid, neat

      For more information please access the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/102/026/protdtbul.pdf

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