MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP).
Application
MCDB-131 Medium has been used:
as a component of the basal medium to culture bovine retinal endothelial cells[1]
to culture bovine lung microvascular endothelial cells[2]
for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells[3]
Quantity
Formulated to contain 11.6 grams of powder per liter of medium.
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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