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L1412

Sigma-Aldrich

Lysing Enzymes from Trichoderma harzianum

lyophilized powder

Synonym(s):

Glucanex®

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About This Item

UNSPSC Code:
12352204

form

lyophilized powder

specific activity

≥10 units/g

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Application

Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
  • for protoplast isolation[1]
  • for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation[2]
  • for the lysis of Aspergillus niger vegetative cells[3]

Biochem/physiol Actions

Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.[4]

Quality

Contains β-glucanase, cellulase, protease, and chitinase activities

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).

Legal Information

A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLBJ0553V
SLBG2959V
SLBB7969V
080M1447V
080M1447

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Influence of protoplast fusion between two Trichoderma spp. on extracellular enzymes production and antagonistic activity
Hassan MM
Biotechnology, Biotechnological Equipment, 28(6), 1014-1023 (2014)
Divya Ramalingam Iyer et al.
PLoS genetics, 13(8), e1006958-e1006958 (2017-08-15)
In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA
Identification of InuR, a new Zn (II) 2Cys6 transcriptional activator involved in the regulation of inulinolytic genes in Aspergillus niger
Yuan XL, et al.
Molecular Genetics and Genomics, 279(1), 11-26 (2008)
Xiao-Lian Yuan et al.
Molecular genetics and genomics : MGG, 279(6), 545-561 (2008-03-06)
The filamentous ascomycete Aspergillus niger is well known for its ability to produce a large variety of enzymes for the degradation of plant polysaccharide material. A major carbon and energy source for this soil fungus is starch, which can be
Cylindrical cellular geometry ensures fidelity of division site placement in fission yeast
Mishra M, et al.
Journal of Cell Science, 125(16), 3850-3857 (2012)
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