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HPA001897

Sigma-Aldrich

Anti-ILF3 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-DRBP76 antibody produced in rabbit, Anti-Double-stranded RNA-binding protein 76 antibody produced in rabbit, Anti-Interleukin enhancer-binding factor 3 antibody produced in rabbit, Anti-NF-AT-90 antibody produced in rabbit, Anti-NFAR antibody produced in rabbit, Anti-Nuclear factor associated with dsRNA antibody produced in rabbit, Anti-Nuclear factor of activated T-cells 90 kDa antibody produced in rabbit, Anti-TCP80 antibody produced in rabbit, Anti-Translational control protein 80 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA001897
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:2500-1:5000

immunogen sequence

VMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAKGLLLKGDLDLELVLLCKEKPTTALLDKVADNLAIQLAAVTEDKYEILQSVDDAAIVIK

UniProt accession no.

Q12906

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ILF3(3609)

General description

ILF3 (interleukin enhancer binding factor 3) is a double-stranded nuclear RNA-binding protein. It is composed of a bipartite nuclear localization signal (NLS), an RGG (Arg-Gly-Gly) box RNA-binding motif, and two double-stranded RNA binding domains (dsRBDs) that acts as novel nuclear export sequence (NES) in intact cells.

Immunogen

Interleukin enhancer-binding factor 3 recombinant protein epitope signature tag (PrEST)

Application

Anti-ILF3 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

Exportin-5 mediates the transport of ILF3 (interleukin enhancer binding factor 3) from nucleus to cytoplasm. VA1 RNA, exportin-5, ILF3 and RanGTP forms a quaternary complex, which mediates the interaction between ILF3 and exportin-5. Later, ILF3 affinity towards VA1 is stimulated by exportin-5 that facilitates the interaction between RNA and the export receptor. These events cause co-export of both VA1 and ILF3.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST86210

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carole Gwizdek et al.
The Journal of biological chemistry, 279(2), 884-891 (2003-10-23)
The karyopherin-related nuclear transport factor exportin-5 preferentially recognizes and transports RNAs containing minihelix motif, a structural cis-acting export element that comprises a double-stranded stem (>14 nucleotides) with a base-paired 5' end and a 3-8-nucleotide protruding 3' end. This structural motif
Matthias Habjan et al.
PLoS pathogens, 9(10), e1003663-e1003663 (2013-10-08)
Viruses that generate capped RNA lacking 2'O methylation on the first ribose are severely affected by the antiviral activity of Type I interferons. We used proteome-wide affinity purification coupled to mass spectrometry to identify human and mouse proteins specifically binding
Amy M Brownawell et al.
The Journal of cell biology, 156(1), 53-64 (2002-01-05)
We have identified a novel human karyopherin (Kap) beta family member that is related to human Crm1 and the Saccharomyces cerevisiae protein, Msn5p/Kap142p. Like other known transport receptors, this Kap binds specifically to RanGTP, interacts with nucleoporins, and shuttles between
Yü Liu et al.
Molecular medicine reports, 20(1), 125-134 (2019-05-23)
Interleukin enhancer‑binding factor 3 (ILF3) may function as a transcriptional coactivator and has been reported to be involved in tumor proliferation and metastasis; however, its role and clinical value in gastric cancer (GC) remains unclear. To understand the value of
Seyedehmahsa Moghimi et al.
PLoS pathogens, 18(10), e1010906-e1010906 (2022-10-29)
As ultimate parasites, viruses depend on host factors for every step of their life cycle. On the other hand, cells evolved multiple mechanisms of detecting and interfering with viral replication. Yet, our understanding of the complex ensembles of pro- and

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