Skip to Content
Merck
All Photos(1)

Documents

GE17-0444-01

Lentil Lectin-Sepharose 4B 25 mL

Cytiva 17-0444-01

Synonym(s):

Sepharose 4B

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

lentil lectin

packaging

pkg of 25 mL

manufacturer/tradename

Cytiva 17-0444-01

storage condition

(20% Ehtanol, PBS pH 7)

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-12

working range

3-10

storage temp.

2-8°C

Related Categories

General description

Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Features and Benefits

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service