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G4410

Sigma-Aldrich

Griess reagent (modified)

Synonym(s):

Greiss, nitrite assay

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About This Item

EC Number:
215-981-2
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

form

powder

Quality Level

200

usage

10 g

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.[1]

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)[2]
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes[3]
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells[4]
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)[5]
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants[6]

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000359170
0000357906
0000351317
0000346347
0000342652

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Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids
Green LC, et al.
Analytical biochemistry, 126(1), 131-138 (1982)
Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes
Pufe T, et al.
Osteoarthritis and Cartilage, 15(2), 155-162 (2007)
Praveen Papareddy et al.
The Journal of biological chemistry, 285(36), 28387-28398 (2010-07-02)
Tissue factor pathway inhibitor (TFPI) inhibits tissue factor-induced coagulation, but may, via its C terminus, also modulate cell surface, heparin, and lipopolysaccharide interactions as well as participate in growth inhibition. Here we show that C-terminal TFPI peptide sequences are antimicrobial
Kazuhiro Ohgami et al.
Investigative ophthalmology & visual science, 44(6), 2694-2701 (2003-05-27)
Astaxanthin (AST) is a carotenoid that is found in marine animals and vegetables. Several previous studies have demonstrated that AST exhibits a wide variety of biological activities including antioxidant, antitumor, and anti-Helicobacter pylori effects. In this study, attention was focused
T M Tikka et al.
Journal of immunology (Baltimore, Md. : 1950), 166(12), 7527-7533 (2001-06-08)
Glutamate excitotoxicity to a large extent is mediated through activation of the N-methyl-D-aspartate (NMDA)-gated ion channels in several neurodegenerative diseases and ischemic stroke. Minocycline, a tetracycline derivative with antiinflammatory effects, inhibits IL-1beta-converting enzyme and inducible nitric oxide synthase up-regulation in
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Questions

1–10 of 11 Questions  

  1. 1. Can the griess reagent solution be stored for longer if I keep it in the freezer? 2. Does my sample (a supernatant of cell culture media) has to be centrifuged first before I do the assay? 3. Do I add the reagent to my standard (NaNO2) as well?

    1 answer

    1. We have no information on storing this product in the freezer. Yes, it is best to spin your cell culture sample down before the assay. Yes, you will need to add the reagent to your standards.

      Helpful?

  2. if we dissolve Griess reagent 40 mg/ml in water then what will be its concentration (molarity).

    1 answer

    1. This product is a multi-component reagent, thus a molarity is not available. When prepared according to instructions, the prepared solution will contain 0.288% (w/v) of sulfanilamide and 0.016% (w/v) naphthylethylene diaminedihydrochloride. In addition, the product also includes an unreported amount of phosphoric acid.

      Helpful?

  3. How many grams of reagent powder should be dissolved in 1 ml of distilled water?

    1 answer

    1. This product is tested for solubility at 40 mg/mL in water. The product is intended to be reconstituted in the bottle by adding 250 ml of water. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdf

      Helpful?

  4. Since this is a solid, how much quantity of water it should be diluted with?

    1 answer

    1. The 10 g quantity is packaged in a 250 mL bottle. To reconstitute, simply add 250 mL of distilled water.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/046/g4410bul.pdf

      Helpful?

  5. how the limit of detection ?

    1 answer

    1. According to T.P. Misko, et al., Analytical Biochemistry, 214, 11-16 (1993), this Griess Reagent, in fluorometric testing, detects to at least 10 nM nitrite. This information has not been validated.

      Helpful?

  6. What is advised for disposal if a solution is made up with H2O?

    1 answer

    1. Please refer to the SDS for disposal information. Per section 13.1, Waste material must be disposed of in accordance with the national and loc No mixing with other waste. Handle uncleaned containers like the product. See www.retrologistik.com for processes regarding the return of chemicals and containers, or contact technical service if you have further questions. https://www.sigmaaldrich.com/sds/sigma/g4410.

      Please contact your EHS team or or disposal service group, If there's none, we recommend a national disposal company such as Cleanharbors at https://www.cleanharbors.com/.

      Helpful?

  7. When using Product G4410, Griess' reagent (modified), what product should I use to make a nitrite standard curve?

    1 answer

    1. Sodium nitrite (Product No. S2252 or 237213) can be used to make a standard curve. A typical standard curve would cover the range of 0-100 microMolar nitrite.

      Helpful?

  8. Is there an enzymatic method to convert nitrate to nitrite?

    1 answer

    1. A coupled assay to convert nitrate to nitrite has been published by Grisham, M.B., et al., Quantitation of nitrate and nitrite in extracellular fluids. Methods Enzymol., 268, 237-246 (1996).  For this protocol, we recommend using Product No. N7265 (Nitrate Reductase), Product No.L1378 (L-Lactic Dehydrogenase), Product No.N1630 (NADPH), Product No. P8574 (Sodium pyruvate) and Product No. F6625 (Flavin adenine dinucleotide disodium salt).

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. Will phenol red interfere with the Griess reaction Product G4410, Griess' reagent (modified)?

    1 answer

    1. While phenol red does not interfere with the Griess reaction, it will interfere with the absorption reading taken at 540nm.  It is recommended to use media without phenol red to avoid any interference in the absorption readings.

      Helpful?

1–10 of 11 Questions  

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