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FLAA

Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit

for ATP quantitation

Synonym(s):

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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1 KIT
€769.00

€769.00

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1 KIT
€769.00

About This Item

EC Number:
213-579-1
UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.26

€769.00

In StockDetails

Request a Bulk Order

Quality Level

200

storage temp.

−20°C

General description

The Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of ATP in samples. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin. When ATP is the limiting reagent, the light emitted is proportional to the ATP present in the sample.

Application

Adenosine 5′-triphosphate (ATP) Bioluminescent Assay Kit has also been used in the quantification of ATP in 3D matrixes of human neurons,[1] hepatic cells with ischemia,[2] various bacterial cultures[3][4] and lysosomes.[5]

Kit Components Only

Product No.
Description
  • FL-AAB Dilution buffer 1 mL/vial
  • FL-AAM Assay mix 1 mL/vial
  • FL-AAS ATP standard 1 mL/vial

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H318

Precautionary Statements

P280 - P305 + P351 + P338

Hazard Classifications

Eye Dam. 1

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ0442
SLCQ0441
SLCP6942
SLCM0389
SLCL1457

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Nadeshda Andreeva et al.
Applied microbiology and biotechnology, 102(12), 5235-5244 (2018-04-24)
Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need
F Chen et al.
Journal of clinical microbiology, 32(11), 2791-2800 (1994-11-01)
A bioluminescent assay which employs the luciferin-luciferase ATP-dependent reaction was used to evaluate the viability of populations of Pneumocystis carinii derived from infected rat lungs. Contamination with host cells was reduced by a purification method which involved a combination of
Yuri L Boteon et al.
PloS one, 14(10), e0224066-e0224066 (2019-10-24)
The combination of hypothermic and normothermic machine perfusion (HMP+NMP) of the liver provides individual benefits of both techniques, improving the rescue of marginal organs. The aim of this study was to investigate the effect on the bioenergetic status and the
Hynek Mergental et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 24(10), 1453-1469 (2018-10-26)
Increased use of high-risk allografts is critical to meet the demand for liver transplantation. We aimed to identify criteria predicting viability of organs, currently declined for clinical transplantation, using functional assessment during normothermic machine perfusion (NMP). Twelve discarded human livers
Evaluation of antimicrobial activities of commercial herb and spice extracts against selected food-borne bacteria
Witkowska A M, et al.
Journal of Food Research, 2(4), 37-37 (2013)
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Questions

1–8 of 8 Questions  

  1. How many times can one do with Product FLAAS, ATP Bioluminescent Assay Kit (one vial of FLAAS) when standard range between 20uM~0.002uM?

    1 answer

    1. The number of times that one can use FLAAS ATP Assay Kit is dependent upon the sample. Frequently, serial dilutions of the sample, as well as the kit reagents, are required. If no dilutions were required the ATP Assay MIx is reconstituted to 5 mL and added to the sample at 0.1 mL. The dilution factor will need to be determined by the end-user. Please note, FLAAS is stable for at least 24 hours at 2–8 °C, or over two weeks if stored frozen at –20 °C. Working concentrations of the serially diluted standard are stable for up to 8 hours when stored in ice.

      Please view the product data sheet for more information, including dilution factor details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/308/573/flaabul.pdf

      Helpful?

  2. How does the kit perform on samples with pH 7.3-7.4 (physiological range)? It recommends adjustment of sample pH to 7.8 which is not always possible.

    1 answer

    1. It is important to use correct pH-value. The luciferase reaction has its pH-optimum in the range of 7.75. Samples having extreme high pH-values or samples of a different pH containing high buffer concentrations may influence the light reaction in an unpredictable way. To avoid issues, correct the pH to a value between 7.6 and 8.0 or dilute samples in Tricine Buffer of the appropriate pH.

      Helpful?

  3. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

    1 answer

    1. When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

      Helpful?

  4. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

    1 answer

    1. With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

      Helpful?

  5. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

    1 answer

    1. This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

      Helpful?

  6. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

    1 answer

    1. One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

      Helpful?

  7. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

    1 answer

    1. The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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