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HFFF2

86031405, human prostate (fetal), Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

HFFF2, 86031405

biological source

human prostate (fetal)

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian foetal foreskin fibroblast

Cell Line Description

Derived from a 14-18 week old human foetus. The cells support growth of CMV and HSV. This cell line has a finite life span.

Application

Virus studies: CMV and HSV

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 8,11
vWA: 16,17

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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