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BHK 21 (Clone 13) from hamster

85011433, hamster kidney, Fibroblast

Synonym(s):

BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells

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About This Item

UNSPSC Code:
41106514

product name

BHK 21 (Clone 13) from hamster, 85011433, from hamster kidney

biological source

hamster kidney

growth mode

Adherent

karyotype

2n = 44, pseudodiploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

Application

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.
Virus replication studies

Culture Medium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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