C8239
Anti-α-N-Catenin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 102 kDa
species reactivity
mouse
technique(s)
microarray: suitable
western blot: 1:1,000 using cytosolic fraction of mouse brain
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Ctnna2(12386)
General description
The catenins (α, β, γ) are cytoplasmic proteins found in varying developing and adult tissues. α- N-catenin is a cadherin-binding protein, that plays a crucial role in cadherin-mediated cell-cell adhesion. It is widely expressed in the nervous system. α-Catenin (CAP102, 102 kDa), originally described as an E-cadherin associated protein, has been shown to associate with N-cadherin and P-cadherin. Within its conserved region α-catenin shows 30% identity to vinculin. There are at least two subtypes of α-catenin: α-E-catenin and α-N-catenin (102 kDa). The predominant form is α-E-catenin. Alternative spliced forms of α-N-catenin include α-N-catenin I and α-N-catenin II. The ratio of the two isoforms changes during development: isoforms II is more abundant than isoform I in early embryonic development, whereas isoform I is predominant in the adult stage.
Immunogen
synthetic peptide corresponding to a region located near the N-terminus of human α-N-catenin (amino acids 171-186) conjugated to KLH. This sequence is identical in mouse and chicken α-N-catenin. It is not found in α-E-catenin, β-catenin, and γ-catenin.
Application
α-N-Catenin antibody is suitable for microarray and western blot at a dilution of 1:1,000 by using cytosolic fraction of mouse brain.
Biochem/physiol Actions
Catenins bind directly or indirectly to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The association of catenins to cadherins produces a complex, which is linked to the actin filament network. Catenins/cadherin complexes play an important role in mediating cell adhesion, transduction of cell-cell contact positional signals to the cell interior, and may play a crucial role in cell differentiation. The expression of α-N-catenin is more restricted and this form predominates in the brain and is localized at synaptic junctions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Unraveling the cytoplasmic interactions of the cadherin superfamily.
P Cowin
Proceedings of the National Academy of Sciences of the United States of America, 91(23), 10759-10761 (1994-11-08)
S Hirano et al.
Cell, 70(2), 293-301 (1992-07-24)
The function of cadherin cell adhesion molecules is thought to be regulated by a group of cytoplasmic proteins, including alpha-catenin. We identified a subtype of alpha-catenin, termed alpha N-catenin, which is associated with N-cadherin and expressed mainly in the nervous
Masato Uemura et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 235(9), 2559-2566 (2006-05-13)
Alpha N-catenin is a cadherin-binding protein, widely expressed in the nervous system; and it plays a crucial role in cadherin-mediated cell-cell adhesion. Here we report the effects of alpha N-catenin gene deficiency on brain morphogenesis. In addition to the previously
D L Rimm et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(19), 8813-8817 (1995-09-12)
Calcium-dependent homotypic cell-cell adhesion, mediated by molecules such as E-cadherin, guides the establishment of classical epithelial cell polarity and contributes to the control of migration, growth, and differentiation. These actions involve additional proteins, including alpha- and beta-catenin (or plakoglobin) and
K Herrenknecht et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(20), 9156-9160 (1991-10-15)
The cytoplasmic region of the Ca(2+)-dependent cell-adhesion molecule (CAM) uvomorulin associates with distinct cytoplasmic proteins with molecular masses of 102, 88, and 80 kDa termed alpha, beta, and gamma catenin, respectively. This complex formation links uvomorulin to the actin filament
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