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C5024

Sigma-Aldrich

Carbonic Anhydrase from bovine erythrocytes

non-denaturing PAGE marker

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

form

lyophilized powder

mol wt

~29 kDa

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

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Application

This product is used as a marker to determine the molecular mass of an unknown protein in non-denaturing electrophoresis systems. In such systems, proteins often retain their biological activity, thus allowing in-gel detection using specific substrate stains. This technique also facilitates the recovery of the active protein. The product yields 3 bands.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuzhu Bian et al.
Artificial cells, nanomedicine, and biotechnology, 41(1), 60-68 (2013-01-26)
Even though erythrocytes transport both oxygen and carbon dioxide, research on blood substitutes has concentrated on the transport of oxygen and its vasoactivity and oxidative effects. Recent study in a hemorrhagic shock animal model shows that the degree of tissue
Barend Andre Stander et al.
PloS one, 7(12), e52205-e52205 (2013-01-10)
Antimitotic compounds are still one of the most widely used chemotherapeutic anticancer drugs in the clinic today. Given their effectiveness against cancer it is beneficial to continue enhancing these drugs. One way is to improve the bioavailability and efficacy by
Nadia Lounnas et al.
Cancer letters, 333(1), 76-88 (2013-01-26)
The membrane-bound carbonic anhydrase isoforms CAIX and CAXII, underpin a pH-regulating system that enables hypoxic tumor cell survival. Here, we observed for the first time an upregulation of CAXII in T-cell acute lymphoblastic leukemia/lymphoma (T-ALL/LL) cells. First we showed that
S Y Liao et al.
British journal of cancer, 108(3), 613-620 (2013-01-10)
Lobular endocervical glandular hyperplasia (LEGH) is a rare lesion of the uterine cervix. It has been proposed that LEGH may represent a precursor lesion to a group of mucinous adenocarcinoma with gastric phenotype (GA) that is independent of high-risk human
Alessio Papi et al.
PloS one, 8(1), e54968-e54968 (2013-02-02)
Cancer stem cell biology is tightly connected to the regulation of the pro-inflammatory cytokine network. The concept of cancer stem cells "inflammatory addiction" leads to envisage the potential role of anti-inflammatory molecules as new anti-cancer targets. Here we report on

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