The form of fetuin has not been determined. This material has not been sequenced. The minimum allowable sialic acid content is 0.5%. The molecular weight is approximately 44,200 daltons. The starting material for this product has a molecular weight of approximately 48,400 including an estimated 8.7% sialic acid. According to the UniPro values for each, the Fetuin-B molecular weight is calculated at 42,663 while the Fetuin-A is 38,419.
A4781
Asialofetuin from fetal calf serum
Type I (Sigma designation)
Synonym(s):
Asialofetuin
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50 MG
€68.30
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€231.00
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€2,190.00
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50 MG
€68.30
250 MG
€231.00
1 G
€2,190.00
About This Item
Recommended Products
biological source
bovine (calf) serum
Quality Level
type
Type I (Sigma designation)
form
powder
impurities
salt, essentially free
≤0.5% N-acetylneuraminic acid
solubility
0.85% sodium chloride: soluble 1 mg/mL
storage temp.
2-8°C
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General description
Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.[1]
Application
Asialofetuin from fetal calf serum has been used:
- to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)[2]
- quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA[3]
- as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins[4]
- to study the nature of the interaction between ferritin and the placenta[5]
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R. [1]
Preparation Note
Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Identification and sequence determination by mass spectrometry have become routine analyses for soluble proteins. Membrane proteins, however, remain challenging targets due to their hydrophobicity and poor annotation. In particular small membrane proteins often remain unnoticed as they are largely inaccessible
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The SDS-glycoprotein system is mimic of membrane protein-lipid system. Fate of glycoprotein, conformation and the interactive forces involved in membrane milieu are expected to be decided by the net charge on glycoprotein that may change during acidic environment in a
Keiichi Motoyama et al.
Journal of drug delivery, 2011, 476137-476137 (2011-04-15)
The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed
R D Lamparelli et al.
British journal of haematology, 72(1), 100-105 (1989-05-01)
The organ distribution of intravenously injected hepatic ferritin either labelled with 59Fe or with 59Fe and 125I, was studied in pregnant guinea-pigs. At 5 h 71.2% of injected 59Fe was present in the placenta and fetus. Transfer of 59Fe to
Antra Ganguly et al.
Current protocols, 1(6), e150-e150 (2021-06-09)
Glycans (oligosaccharide chains attached to glycoproteins) are a promising class of biomarkers, found in body fluids such as serum, saliva, urine, etc., that can be used for the diagnosis of disease conditions. Subtle changes in glycans resulting from altered glycosylation
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To wich fetuin corresponds your product A4781 (Asialofetuin from fetal calf serum). Is it Protein Fetuin-A ? Uniprot entry: P12763 FETUA_BOVIN or protein Fetuin-B ? Uniprot entry: Q58D62 FETUB_BOVIN Do you have de amino acid sequence?
1 answer-
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Hi, What is the difference in Type 1 versus Type II asialofetuin
1 answer-
The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, uses a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
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