A4227

Anti-ASAP1/Centaurin β4 (ED-20) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Arf GAP containing SH3, ANK repeat, and PH domains/Centaurin β4 (ED-20), DEF1
• MDL number: MFCD04040184
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 130 kDa (doublet band)
• species reactivity: rat, mouse, human
• technique(s): immunofluorescence: 1:1,000 using mouse NIH3T3 fibroblast cells; microarray: suitable; western blot: 1:2,500 using rat brain and mouse brain extracts
• UniProt accession no.: Q9ULH1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ASAP1(50807) ; mouse ... Asap1(13196) ; rat ... Asap1(314961)

Description

General description

ASAP1 is a phospholipid-dependent ARF GTPase-activating protein that activates ARF for membrane trafficking regulation.

Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody recognizes human ASAP1 by immunoblotting (130kDa, doublet band). Staining of ASAP1 in immunoblotting is specifically inhibited with the ASAP1 immunizing peptide.

Immunogen

synthetic peptide located near the C-terminus of mouse ASAP1 (amino acids 1128-1147) conjugated to KLH.

Application

Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody can be used for immunofluorescence (1:1,000 using mouse NIH3T3 fibroblast cells) and western blot (1:2,000 using rat brain and mouse brain extracts) assays. It is also suitable for use in microarray applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable