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18502

Millipore

Oxidase Reagent according to Gordon-McLeod

suitable for microbiology

Synonym(s):

Gordon-McLeod Reagent

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

Agency

according to ISO 9308-1:2014

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

N,N-dimethyl-p-phenylenediamine hydrochloride, 0.15 g
water, 10 mL

packaging

pkg of 100 mL

storage condition

(Tightly closed)

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Plesiomonas spp.
Aeromonas spp.
Neisseria spp.
Pseudomonas spp.
bacteria

Related Categories

General description

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory reagent in microbiology to detect bacteria that produce cytochrome c oxidase. The reagent is designed to identify this enzyme′s presence, which plays a critical role in the electron transport chain and indicates respiratory metabolism in bacteria. The Gordon-McLeod reagent contains N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride, as the active component in the reagent. When an organism produces cytochrome c oxidase, the reagent undergoes a color change reaction, turning from colorless to blue or purple. This color change is a positive result, indicating the presence of cytochrome c oxidase activity in the tested organism.

Application

Oxidase Reagent, according to Gordon-McLeod, is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce cytochrome c oxidase. The Gaby-Hadley A reagent provides a simple and efficient way to identify oxidase-positive bacteria, such as Pseudomonas, Vibrio, and some Neisseria species, while producing negative results for organisms that lack cytochrome c oxidase activity. in the food, beverage, and pharmaceutical industries.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM3467
BCCK1992
BCCJ4482
BCCG2223
BCCD0287

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Articles

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Campylobacter Detection and Identification

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

Media for Staphylococcus Aureus Detection

Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.

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Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

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