11119915001
Roche
RNase, DNase-free
from bovine pancreas
Synonym(s):
Rnase
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About This Item
UNSPSC Code:
41105600
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biological source
bovine pancreas
Quality Level
form
solution
specific activity
≥30 units/mg protein
packaging
pkg of 500 μg (1 ml)
manufacturer/tradename
Roche
technique(s)
DNA purification: suitable
storage temp.
−20°C
Related Categories
General description
Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.
Application
RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.
Unit Definition
One Kunitz unit is the amount of enzyme that causes a decrease in absorbance of A0 to A1 within one minute under the assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.
Physical form
Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (pH 7.0).
Preparation Note
Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below:
Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.
- For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
- To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
- To isolate genomic DNA from cultured mammalian cells (5 x 107 cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
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Protocols
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
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