MABS489

Anti-LRP-2/Megalin Antibody, clone 10D5.1

clone 10D5.1, from mouse

• Synonym(s): Low-density lipoprotein receptor-related protein 2, Calcium sensor protein, Glycoprotein 330, gp330, LRP-2, Megalin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10D5.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_004516
• UniProt accession no.: P98164
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LRP2(4036)

Description

General description

Low-density lipoprotein receptor-related protein 2 (UniProt P98164; also known as Calcium sensor protein, Glycoprotein 330, gp330, Heymann nephritis antigen homolog, LRP-2, Megalin) is encoded by the LRP2 (also known as DBS) gene (Gene ID 4036) in human. Megalin/LRP-2 is a 600 kDa type I membrane receptor initially produced with a signal peptide sequence (a.a. 1-25). The mature receptor has a large extracellular region (a.a. 26-4423), a transmembrane segment (a.a. 4424-4446), and cytoplasmic tail (a.a. 4447-4655). Megalin belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the very-low-density lipoprotein (VLDL) receptor, the LDLR-related protein (LRP), and the apolipoprotein E receptor 2 (ApoER2). The overall extracellular structural features are similar among all LDLR family members. Megalin contains four clusters of cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding. These are separated by 17 EGF-like repeats and eight cysteine-poor spacer regions that contain YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments. Megalin is a multiligand, endocytic receptor expressed primarily in the apical membranes of polarized epithelial cells. In addtion to functioning alone, megalin is also reported to interact with another structurally distinct endocytic receptor cubilin in several tissues where they are co-expressed. The internalization of several cubilin ligands is reported to be strongly facilitated in the presence of megalin.

Immunogen

Epitope: LDL-receptor class A repeats 19-21.

GST-tagged recombinant protein corresponding to the LDL-receptor class A repeats 19-21 of human LRP-2/Megalin.

Application

Research Category
Signaling

Research Sub Category
Lipid Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected LRP-2/Megalin in 10 µg of human kidney tissue lysate.

Quality

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LRP-2/Megalin in human kidney tissue.

Target description

~250 to 600 kDa observed. A broad/smeared banding pattern is consistent with the detection of the target protein with varying degrees of glycosylation (520 kDa/non-glycosylated to 660 kDa/fully glycosylated), as well as smaller size degradation products.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable