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antibody form
purified antibody
clone
AGP4
monoclonal
purified by
ion-exchange chromatography
species reactivity
mouse, all, all
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgMκ
General description
Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
Specificity
Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).
Application
Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).
Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).
ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).
ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
Quality
Evaluated by ELISA with amino- and methoxy PEG molecules (10K-PEG, 3K-PEG, and 2K-mPEG).
ELISA Analysis: 5 µg/mL of this antibody detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG).
ELISA Analysis: 5 µg/mL of this antibody detected amino- and methoxy-PEG molecules (10K amino-PEG, 3K-amino PEG, and 2K-methoxy PEG).
Physical form
Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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