ABE577

Anti-Myb Binding Protein 1A Antibody

serum, from rabbit

• Synonym(s): Myb-binding protein 1A
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ChIP, ICC, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): ChIP: suitable, immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_001099008
• UniProt accession no.: Q9BQG0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MYBBP1A(10514)

Description

General description

Myb-binding protein 1A (MYBBP1A) interacts with sequence specific DNA-binding proteins in order to activate or repress transcription. Transcriptional repression via Myb-binding protein 1A is thought to be mediated by histone deacetylase activity (HDAC activity).

~160 kDa observed. Uncharacterized band(s) may be observed in some cell lysates. The full length of this protein is observed at ~160 kDa; however, it is proteolytically cleaved into ~140 kDa and ~67 kDa fragments (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.; Mori, S., et al. (2012). PLoS One. 7(10):e39723.).

Immunogen

GST-tagged recombinant protein corresponding to human Myb Binding Protein 1A.

Application

Anti-Myb Binding Protein 1A Antibody is a highly specific rabbit polyclonal antibody, that targets Myb-binding protein 1A & has been tested in western blotting & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Myb Binding Protein 1A in diploid human lung embryonic fibroblasts (Prof. Atilla Nemeth, University of Regensburg, Germany.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Myb Binding Protein 1A in HeLa cells (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated Myb Binding Protein 1A in ChIP (Hochstatter, J., et al. (2012). J Biol Chem. 287(29):24365-24377.).

Biochem/physiol Actions

This antibody detects the full length and cleaved forms of Myb Binding Protein 1A.

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analyis: A 1:1,000 dilution from a representative lot detected Myb Binding Protein 1A in 10 µg of A431 cell lysate.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1