373272

HECAMEG

Non-ionic detergent useful for solubilization of membrane-bound proteins in their native state. Easily removed by dialysis.

• Synonym(s): Methyl-6-O-(N-heptylcarbamoyl)-α-D-glucopyranoside
• Empirical Formula (Hill Notation): C₁₅H₂₉NO₇
• CAS Number: 115457-83-5
• Molecular Weight: 335.39
• MDL number: MFCD00077425
• UNSPSC Code: 12161902
• NACRES: NA.21

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• CMC: 19.5 mM
• solubility: water: 10 mg/mL
• shipped in: ambient
• storage temp.: 15-25°C
• InChI: 1S/C15H29NO7/c1-3-4-5-6-7-8-16-15(20)22-9-10-11(17)12(18)13(19)14(21-2)23-10/h10-14,17-19H,3-9H2,1-2H3,(H,16,20)/t10-,11-,12+,13-,14+/m1/s1
• InChI key: XPIVOYOQXKNYHA-RGDJUOJXSA-N

Description

General description

Non-ionic detergent useful for solubilization of membrane-bound proteins in their native state. Easily removed by dialysis.

Nonionic detergent useful for solubilization of membrane-bound proteins in their native state. Easily removed by dialysis. Has a CMC of 19.5 mM.

Application

• An assessment of the biochemical applications of the non-ionic surfactant Hecameg.: This foundational study explores the biochemical applications of Hecameg, particularly its efficacy in solubilizing membrane proteins without denaturation, making it a valuable tool in membrane biochemistry research (Ruiz et al., 1994).


• Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii.: Hecameg is utilized in this study for the purification of the cytochrome b6 f complex, demonstrating its effectiveness in maintaining the functional integrity of membrane protein complexes during isolation (Pierre et al., 1995).


• Development of an improved in vitro activity assay for medium chain length PHA polymerases based on CoenzymeA release measurements.: This article describes the use of Hecameg in an improved assay for polymerase activity, emphasizing its role in biochemical methods that require the stabilization of enzyme activities (de Roo et al., 2000).

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Plusquellec, D., et al. 1989. Anal. Biochem.179, 145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable