361554

TWS119

≥95% (HPLC), solid, GSK-3β inhibitor, Calbiochem^®

• Synonym(s): GSK-3β Inhibitor XII, TWS119, Neurogenesis Inducer, TWS119
• Empirical Formula (Hill Notation): C₁₈H₁₄N₄O₂
• CAS Number: 601514-19-6
• Molecular Weight: 318.33
• MDL number: MFCD09037535
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: GSK-3β Inhibitor XII, TWS119, The GSK-3β Inhibitor XII, TWS119, also referenced under CAS 601514-19-6, controls the biological activity of GSK-3β. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• potency: 30 nM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: beige
• solubility: methanol: 2 mg/mL; DMSO: 200 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C18H14N4O2/c19-12-4-1-3-11(7-12)16-9-15-17(22-16)20-10-21-18(15)24-14-6-2-5-13(23)8-14/h1-10,23H,19H2,(H,20,21,22)
• InChI key: VPVLEBIVXZSOMQ-UHFFFAOYSA-N

Description

General description

A cell-permeable and potent inhibitor of GSK-3β (IC₅₀ = 30 nM). Binds to GSK-3β with high-affinity (K_d = 126 nM), leading to an increase in the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).

A cell-permeable pyrrolopyrimidine compound that acts as a potent inhibitor of GSK-3β (IC₅₀ = 30 nM). Binds to GSK-3β with high-affinity (K_d = 126 nM) and increases the level of β-catenin, a downstream substrate of GSK-3β in the Wnt signaling pathway. Shown to selectively induce neuronal differentiation in both pluripotent murine embryonal carcinoma cells (P19; 30-40% at 1 µM) and embryonic stem cells (D3; 50-60% at 400 nM).

Biochem/physiol Actions

Cell permeable: yes

Kd = 126 nM against GSK-3β

Primary Target
GSK-3β

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ding, S., et al. 2003. Proc. Natl. Acad. Sci. USA100, 7632.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3