203637-M
Anti-Bone Sialoprotein Mouse mAb (ID1.2)
lyophilized, clone ID1.2, Calbiochem®
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About This Item
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
ID1.2, monoclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
0.04% Thimerosal as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Anti-Bone Sialoprotein, mouse monoclonal, clone ID1.2, recognizes ~70-80 kDa bone sialoprotein in osteoblasts and osteocytes. It is validated for ELISA, Western blotting, and for paraffin sections.
Purified mouse monoclonal antibody. Recognizes the ~70-80 kDa bone sialoprotein.
Recognizes the ~70-80 kDa bone sialoprotein in osteoblasts and osteocytes.
Immunogen
Human
purified human bone sialoprotein
Application
ELISA (see comments)
Immunoblotting (1:1000)
Paraffin Sections (see comments)
Immunoblotting (1:1000)
Paraffin Sections (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 50 mM Tris, pH 7.4.
Reconstitution
Reconstitute with dH₂O. Please see label for lot-specific reconstitution volume.
Analysis Note
Positive Control
Human osteoblasts or osteocytes
Human osteoblasts or osteocytes
Other Notes
Does not cross-react with human osteocalcin, osteonectin, osteopontin, or bone alkaline phosphatase. This antibody has also been reported to work for ELISA and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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