178251

α₁-Antitrypsin, Human Plasma

α₁-Antitrypsin, CAS 9041-92-3, is a protease inhibitor of the serpin superfamily. Inhibits elastase, cathepsin G, and proteinase 3.

• Synonym(s): α₁-Antitrypsin, Human Plasma, α₁-AT, α₁-Proteinase Inhibitor, 3.5S α₁-Glycoprotein
• CAS Number: 9041-92-3
• MDL number: MFCD00130469
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (SDS-PAGE)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A single polypeptide chain of 394 amino acids and three carbohydrate side chains linked to asparagine residues present at about 290 mg/100 ml in acute phase plasma. Serine protease inhibitor that also acts as a major physiological regulator of elastase. Inhibitor of neutrophil elastase, cathepsin G, and proteinase 3.

A single polypeptide chain of 394 amino acids and three carbohydrate side chains linked to asparagine residues. Present at about 290 mg/100 ml in acute phase plasma. Serine protease inhibitor that also acts as a major physiological regulator of elastase.

Biochem/physiol Actions

Cell permeable: no

Primary Target
serine proteases

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 300 mM NaCl, 30 mM sodium phosphate buffer, pH 6.5.

Preparation Note

Please refer to vial label for lot-specific volume prior to lyophilization.

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Analysis Note

>=75% inhibition with active-site titrated porcine trypsin using benzoyl-Arg-p-nitroanilide (Bz-Arg-pNA) as the substrate.

Other Notes

Coakley, R.J., et al. 2001. Am. J. Med. Sci.321, 33.
Travis, J., and Salvesen, G.S. 1983. Annu. Rev. Biochem.52, 655.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable