1.52371

Chromolith^® WP 300 RP-18

L × I.D. 50 mm × 2 mm HPLC column

• Synonym(s): Chromolith^® WP 300 RP-18 50-2 HPLC column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• Quality Level: 100
• feature: endcapped: no
• extent of labeling: 18% loading
• parameter: 200 bar pressure; 60 °C max. temp.
• technique(s): HPLC: suitable; mass spectrometry (MS): suitable
• column L × I.D.: 50 mm × 2 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 2 μm macroporosity; 30 mm mesoporosity
• operating pH range: 2-7.5

Description

General description

The most hydrophobic of the Chromolith^® WP 300 line, the RP-18 column is useful for the resolution of peptides and smaller proteins. One critical quality attribute (CQA) required by regulatory bodies is the peptide map of a biotherapeutic. Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RPHPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information.
The Chromolith^® WP 300 RP-18 column gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry incorporated into the Chromolith^® WP 300 line improve resolution by increasing efficiency and reducing peak tailing.

Chromolith^® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith^® WP 300 RP-18 2 mm I.D. columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), the surface modification is Octadecylsilane. Chromolith^® WP 300 RP-18 columns fall under the USP L1 Classification. Chromolith^® WP 300 RP-18 columns are fully suitable for LC-MS applications.

The column dimension of 50 mm length and 2 mm I.D. is the best choice for very fast separations of Biomolecules on any UHPLC System.
Chromolith^® WP 300 RP-18 50-2 HPLC columns enable:
—Completely bioinert column hardware and high biorecovery
—High-speed separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® WP 300 RP-18, 5-2 guard cartridges (3 pieces) [1.52372] with the Chromolith^® Guard Cartridge Holder Bioinert 5 mm x 2 mm [1.52355]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series. [[1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany