14-448-M

Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• product line: Upstate^®
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol wt: M_w 35 kDa
• purified by: (The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• accession no.: (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. ); NM_001798.2
• NCBI accession no.: NM_001798.2; NM_052827.1
• UniProt accession no.: P24941

Description

General description

C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A

Biochem/physiol Actions

Protein Target: CDK2/cyclinA

Target Sub-Family: CMGC

Packaging

Also available in 10 μg size and in bulk

Also available in 250μg size, please inquire for pricing and availability.

Quality

routinely evaluated by phosphorylation of Histone H1

Target description

CDK2/cyclinA

Linkage

Replaces: 14-407

Physical form

The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose^™ and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(C): Not applicable