Osmium tetroxide for microscopy can be used to visualize unsaturated lipids. A part of the lipids is immediately blackened by the influence of osmic acid solution. The immediate black coloration results from the -C=C- double bond of the unsaturated fatty acid. The fatty acids that are made brownish at first can be blackened by treatment with 60-70% alcohol. For electron microscopy or semithin sectioning the glutardialdehyde-fixed specimens are post-fixed with osmium(VIII) oxide. Lipid inclusions appear black on a yellow to brown background. It is an IVD / CE registered product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 1
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