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07-1387

Sigma-Aldrich

Anti-oxidized-CaM Kinase II (Met281/282) Antibody

serum, from rabbit

Synonym(s):

CAMKK beta protein, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta, Calcium/calmodulin-dependent protein kinase kinase beta, calcium/calmodulin-dependent protein kinase beta, calcium/calmodulin-dependent protein kinase kinase 2 beta, calcium/calmodulin-

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_742127

UniProt accession no.

Q96RR4

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CAMK2D(817)

General description

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

Specificity

Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.
This antibody recognizes CaM Kinase II when oxidized on Met281/282.

Immunogen

Epitope: Oxidized Met281/282
Synthetic peptide encompassing and including oxidized Met281/282.

Application

An independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).
This Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).

Quality

Evaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.

Target description

60 kDa

Linkage

Replaces: 04-1079

Storage and Stability

Stable for 1 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yao Wu et al.
BioMed research international, 2017, 4150158-4150158 (2017-05-26)
Background. This study was undertaken to determine relative contributions of phosphorylation and oxidation to the increased activity of calcium/calmodulin-stimulated protein kinase II (CaMKII) in juveniles with cardiac myocyte dysfunction due to increased pressure overload. Methods. Juvenile rats underwent abdominal aortic
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Airway epithelial cells play important roles in allergic asthma. Transient receptor potential melastatin-related 2 (TRPM2) and oxidized Ca2+/calmodulin-dependent protein kinase Ⅱ (ox-CaMKⅡ) participate in the airway inflammation. This study aimed to analyze the effects of TRPM2 on ox-CaMKⅡ in the
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Occlusal disharmony is known to affect not only the oral cavity environment, but also the autonomic nervous system in the heart. Since the renin-angiotensin system (RAS) inhibitor captopril (Cap) is one of the first-line drugs for preventing cardiac remodeling in
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