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S7656

Sigma-Aldrich

Anti-Staphylococcal Enterotoxin A antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-Ent A

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About This Item

MDL number:
MFCD00082016
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

Staphylococcus aureus

technique(s)

dot blot: 1:20,000 using using purified Staphyloccal Enterotoxin A immobilized on nitrocellulose membranes (protein concentation: 50 ng/dot).
indirect ELISA: 1:20,000 using using Staphylococcal enterotoxin A

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The antibody is positive against Staphylococcal enterotoxin A, negative versus Staphylococcal enterotoxin B, Cholera toxin, and Pseudomonas exotoxin A (protein concentration: 50-500 ng/dot). The antibody has not been tested for neutralization potency against active Staphylococcal enterotoxin A.

Immunogen

enterotoxin A from Staphylococcus aureus.

Application

Anti-Staphylococcal Enterotoxin A antibody has been used in western blotting, and enterotoxin A detection by ELISA (enzyme-linked immunosorbent assay).
Anti-Staphylococcal Enterotoxin A antibody produced in rabbit was used as a control to SEA-conjugated MUSE11 antibody produced in xenografted SCID mice.

Biochem/physiol Actions

Staphylococcal enterotoxin A (SEA) from Staphylococcal aureus is leading agent that causes of food poisoning. A concentration of as little as 0.5 mg/ml is sufficient to result in nausea, vomiting, diarrhea and cramps. It stimulates the cell proliferation of peripheral lymphocytes, induces the production of interferons and is important for gut immunity against S. aureus infections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma
Takemura S I, et al.
Cancer Immunology, Immunotherapy, 51(1), 33-44 (2002)
Molecular Screening of Staphylococcal Enterotoxin Type A Encoding Gene from MRS Clinical Isolates
Hussein H A, et al.
American Journal of Microbiological Research, 4(2), 68-72 (2016)
M Shinoda et al.
Cancer research, 58(13), 2838-2843 (1998-07-14)
To reinforce cytotoxic activity and the targeting ability of lymphokine-activated killer cells with a T-cell phenotype (T-LAK) for adoptive immunotherapy against human bile duct carcinoma (BDC), staphylococcal enterotoxin A (SEA) was conjugated chemically with MUSE11 monoclonal antibody (MUSE11 mAb), directed
Nicoletta Schwermann et al.
PLoS pathogens, 19(12), e1011892-e1011892 (2024-01-02)
Staphylococcus aureus is a dangerous pathogen that evolved refined immuno-evasive strategies to antagonize host immune responses. This involves the biogenesis of death-effector deoxyribonucleosides, which kill infectious foci-penetrating macrophages. However, the exact mechanisms whereby staphylococcal death-effector deoxyribonucleosides and coupled imbalances of
Yangli Wan et al.
Foods (Basel, Switzerland), 10(12) (2021-12-25)
Foodborne Staphylococcus aureus (S. aureus) has attracted widespread attention due to its foodborne infection and food poisoning in human. Shikonin exhibits antibacterial activity against a variety of microorganisms, but there are few studies on its antibacterial activity against S. aureus.
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