Based on an approximate molecular weight of 12,600, this block co-polymer with two hydroxyl groups has a theoretical hydroxyl value of 8.9 mg potassium hydroxide (KOH) per gram of polymer. Higher experimental results for the hydroxyl value would indicate some branching.
P2443
Pluronic® F-127
powder, BioReagent, suitable for cell culture
Synonym(s):
Non-ionic copolymer surfactant
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About This Item
Linear Formula:
(C3H6O·C2H4O)x
CAS Number:
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.75
Pricing and availability is not currently available.
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description
Non-ionic
contains 100ppm BHT
Quality Level
product line
BioReagent
form
powder
mol wt
~12600 g/mol
technique(s)
cell culture | mammalian: suitable
CMC
950-1000 ppm (~25°C)
transition temp
cloud point >100 °C
HLB
18 - 23
SMILES string
O2C(C2)C.O1CC1
InChI
1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2
InChI key
RVGRUAULSDPKGF-UHFFFAOYSA-N
Related Categories
General description
Pluronicpluronic® F-127, also known as poloxamer 407, is a non-toxic[1] and nonionic copolymer. It contains polyethylene oxide-polypropylene oxide arranged in a triblock structure to form PEO-PPO-PEO symmetric.[2] Surfactant properties of this polymer derived from its amphiphilic nature, allow them to interact with hydrophobic surfaces and biological membranes. [3]
Application
Pluronic® F-127 has been used:
- as a delivery system for a peptide to the wound site to study the effect of the peptide on wound healing in mice.[4]
- as a surface coating for PDMS (polydimethylsiloxane) stamps to make them hydrogel repellent.[5]
- in the preparation of calcium indicator stock solution for measuring neural activities.[6]
- as a component of phosphate-buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.[7]
- This non-ionic copolymer surfactant is suitable for use in insect cell culture applications as an antifoaming agent.
Biochem/physiol Actions
Pluronic® F-127 is a thermo-reversible gel that finds its application in different industries due to its high thermosensitivity, biodegradability, and biocompatibility properties.[1] These properties make this polymer suitable for use as a versatile drug carrier.Pluronic® F-127 improves the therapeutic efficiency of drugs by increasing their bioavailability and thermostability.[8] Additionally, it can promote cellular attachment and collagen formation, which facilitates angiogenesis and tissue regeneration. Due to its efficacy, Pluronic F-127 has been utilized in the encapsulation of mesenchymal stem cells (MSCs) to aid in the regeneration of tissues with low levels of vascularity, such as epithelial tissues, adipose tissues, cartilage, tendons, and bone tissues.[1]
Legal Information
Pluronic is a registered trademark of BASF
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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What is the hydroxyl number of F127?
1 answer-
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Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?
1 answer-
There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:
Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756
Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162
Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853
These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.
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I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?
1 answer-
The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:
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can I know what is the chain length of each PEO and PPO of this F-127?
1 answer-
Pluronic F 127 corresponds to the structure of Kolliphor P 407.
This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
A = 101
B = 56Helpful?
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What is the VOC content of this product?
1 answer-
Volatile Organic Compound (VOC) content is not measured for this product.
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Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?
1 answer-
The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.
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What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?
1 answer-
The solution can be sterilized with a 0.22 uM sterile filter.
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Is corn oil soluble in Pluronic PBS gel?
1 answer-
In general, Pluronic acid products are poor solubilizers of oils. However, they may make a good dispersant or emulsifier for corn oil.
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Is Pluronic® F-127 the same chemical as Poloxamer 407 (16758-250G)? CAS appears to be the same
1 answer-
Yes, Pluronic® F-127 is the same chemical as Poloxamer 407 as stated under Depositor Supplied
Synonyms on PubChem are available at this link: https://pubchem.ncbi.nlm.nih.gov/compound/24751#section=Depositor-Supplied-Synonyms.
Kindly review each of the specifications and tested applications for each product before making a purchasing decision.Helpful?
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Why has my solution of Pluronic F-127, Product P2443, gelled?
1 answer-
Pluronic F-127 will form gels above 10 °C when used at concentrations of 18-50%. It will re-liquefy when cooled to below 10 °C. The gel should remain stable during autoclaving.
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