M3769

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: no; phenol red: no; HEPES: no; sodium pyruvate: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
• to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
• to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Quantity

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable