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Key Documents

L1394

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

agarose conjugate

Quality Level

form

saline suspension

extent of labeling

~6 mg per mL

matrix

4% agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

particle size

45-165 μm

capacity

≥0.5 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

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Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Physical form

Suspension in 0.9% NaCl containing 15 mM Sodium azide.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The
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