L0259

Lucifer Yellow CH dilithium salt

Powder

• Synonym(s): 6-Amino-2,3-dihydro-1,3-dioxo-2-hydrazinocarbonylamino-1H-benz[d,e]isoquinoline-5,8-disulfonic acid dilithium salt
• Empirical Formula (Hill Notation): C₁₃H₉Li₂N₅O₉S₂
• CAS Number: 67769-47-5
• Molecular Weight: 457.25
• Beilstein: 4644601
• EC Number: 267-047-9
• MDL number: MFCD00006922
• UNSPSC Code: 12171500
• PubChem Substance ID: 24896250
• NACRES: NA.47

Properties

• product name: Lucifer Yellow CH dilithium salt, fluorescent stain
• form: powder
• color: faint orange to very dark orange
• solubility: H₂O: 1 mg/mL
• fluorescence: λ_ex 428 nm; λ_em 540 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Li+].[Li+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C13H11N5O9S2.2Li/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2
• InChI key: RPKCZJYDUKVMGF-UHFFFAOYSA-L

Description

General description

Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. Lucifer Yellow CH dilithium salt is a fluorescent dye(1) comprising of amino, sulfo and phenyl-N-hydrazinocarbonylamino substituents on the naphthalimide chromophore. The hydrophilic nature of Lucifer yellow CH underlies its numerous applications as a stain of living cells and tissues.

Application

Lucifer yellow CH has been used to stain nerve cells and to demonstrate electrical coupling between hamster oocytes and cumulous cells during meiotic maturation in vivo and in vitro.
Lucifer Yellow CH is mainly used as a tracer dye and useful in marking nerve cells.

A highly fluorescent dye, useful in marking nerve cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves