D1821
DAF-FM
≥98% (HPLC)
Synonym(s):
4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM
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About This Item
Empirical Formula (Hill Notation):
C21H14F2N2O5
CAS Number:
Molecular Weight:
412.34
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.77
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Quality Level
Assay
≥98% (HPLC)
form
powder
storage condition
desiccated
color
white to brown
solubility
DMSO: >1 mg/mL
shipped in
wet ice
storage temp.
−20°C
SMILES string
CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35
InChI
1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChI key
DIJCILWNOLHJCG-UHFFFAOYSA-N
Application
DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.
Biochem/physiol Actions
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
Caution
hygroscopic
Preparation Note
Color variation within the range from white to brown does not change performance.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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