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Key Documents

C2724

Sigma-Aldrich

Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CALB, Anti-D-28K

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 28 kDa

species reactivity

rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry: 1:2,000 using sections of rat cerebellum
western blot: 1:2,000 using a whole extract of rat brain

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21.
Calbindin-D-28k is an intracellular protein characterized by EF-hand type structural motifs that bind to Ca2+. This protein modulates synaptic plasticity and signal transduction . Anti-Calbindin-D-28K (EG-20) antibody recognizes rat calbindin-D-28K (28 kDa). Due to interspecies similarity, reactivity with human, mouse, bovine, chicken, porcine, and frog is expected, but has not been confirmed. Staining of calbindin-D-28K is specifically inhibited with the calbindin-D-28K immunizing peptide by immunoblotting.

Immunogen

The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences.
synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH.

Application

Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody.
Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC) peroxidase staining and cupric silver staining. It has also been used in immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Analysis Note

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Long-term decrease in calbindin-D28K expression in the hippocampus of epileptic rats following pilocarpine-induced status epilepticus
Carter D S, et al.
Epilepsy Research, 79(2-3), 213-223 (2008)
Postnatal phencyclidine administration selectively reduces adult cortical parvalbumin-containing interneurons
Wang C Z, et al.
Neuropsychopharmacology, 33(10), 2442-2442 (2008)
GABA and synaptic inhibition of mouse cerebellum lacking glutamate decarboxylase 67
Obata K, et al.
Biochemical and Biophysical Research Communications, 370(3), 429-433 (2008)
S Yang et al.
Cell death & disease, 5, e1217-e1217 (2014-05-09)
Diabetic neuropathy develops on a background of hyperglycemia and an entangled metabolic imbalance. There is increasing evidence of central nervous system involvement in diabetic neuropathy and no satisfactory treatment except maintenance of good glycemic control, thereby highlighting the importance of
Hartmut Schmidt
Frontiers in molecular neuroscience, 5, 25-25 (2012-03-22)
Many neurons of the vertebrate central nervous system (CNS) express the Ca(2+) binding protein calbindin D-28k (CB), including important projection neurons like cerebellar Purkinje cells but also neocortical interneurons. CB has moderate cytoplasmic mobility and comprises at least four EF-hands

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