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Fluo 3-AM solution

Name: Fluo 3-AM solution
Brand: SIGMA

1 mM in DMSO, ≥99.0% (TLC)

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About This Item

Empirical Formula (Hill Notation):

C₅₁H₅₀Cl₂N₂O₂₃

CAS Number:

121714-22-5

Molecular Weight:

1129.85

MDL number:

MFCD00083336

UNSPSC Code:

12171500

NACRES:

NA.32

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Properties

Quality Segment

100

assay

≥99.0% (TLC)

form

liquid

concentration

1 mM in DMSO

fluorescence

λ_ex 506 nm; λ_em 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase

storage temp.

−20°C

SMILES string

Clc1[c](cc2[o]c3c(c(c2c1)c4cc(c(cc4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOc5c(ccc(c5)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)cc(c(c3)OCOC(=O)C)Cl)=O

InChI

1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2

InChI key

PTPUOMXKXCCSEN-UHFFFAOYSA-N

Description

General description

Cell permeable fluorescent indicator of intracellular Ca²⁺; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca²⁺; lower binding affinity allows measurement of higher peaks of Ca²⁺ transients than with Fura 2.

Application

suitable as fluorescent Ca sensitive probe (after cleavage of the AM ester)

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This Item	344906	47989	344905
Sigma-Aldrich 39294 Fluo 3-AM solution	Sigma-Aldrich 344906 FURA 2/AM in Solution	Sigma-Aldrich 47989 Fura 2-AM	Sigma-Aldrich 344905 FURA 2/AM
description 1 mM in DMSO, ≥99.0% (TLC)	description -	description BioReagent, suitable for fluorescence, ≥95.0% (HPLC)	description Cell-permeable ester form of FURA 2.
form liquid	form liquid	form solid	form solid
assay ≥99.0% (TLC)	assay ≥95% (HPLC)	assay ≥95.0% (HPLC)	assay ≥95% (HPLC)
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
concentration 1 mM in DMSO	concentration -	concentration -	concentration -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
fluorescence λ_ex 506 nm; λ_em 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase	fluorescence λ_ex 335 nm (high), λ_em 505 nm (high), λ_em 512 nm (low), λ_ex 362 nm (low)	fluorescence λ_ex 355 nm; λ_em 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca²⁺	fluorescence λ_ex 362 nm (low), λ_em 505 nm (high), λ_em 512 nm (low), λ_ex 335 nm (high)

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C

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