X114

Triton^™ X-114

laboratory grade

• Synonym(s): (1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: (C₂H₄O)n C₁₄H₂₂O, n = 7 or 8
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• UNSPSC Code: 12161900
• PubChem Substance ID: 24902129
• NACRES: NA.21

Properties

• grade: laboratory grade
• Quality Level: 200
• description: non-ionic
• mol wt: average mol wt 537
• composition: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x = 7-8
• refractive index: n20/D 1.488 (lit.)
• CMC: 0.2 mM (20-25°C)
• transition temp: pour point ~−9 °C; cloud point 23 °C
• density: 1.058 g/mL at 25 °C (lit.)
• HLB: 12.4
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton^™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton^™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton^™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton^™ X-114 has been used:

• To remove endotoxin from solutions of protein using phase separation technique
• In the extraction and purification of DNA from endotoxin
• In the biphasic extraction of lipoproteins from bacterial cell wall
• As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 483.8 °F - closed cup
• Flash Point(C): 251 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves