S4306
Spirit blue agar
NutriSelect® Plus, suitable for microbiology
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About This Item
UNSPSC Code:
41171606
NACRES:
NA.85
Recommended Products
sterility
non-sterile
Quality Level
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | specific enzyme detection: suitable
microbiological culture: suitable
final pH
6.8±0.2 (25 °C)
application(s)
bioburden testing
cleaning products
cosmetics
food and beverages
personal care
microbiology
suitability
lipolytic bacteria
nonselective and differential for Staphylococcus spp.
Application
Used for the detection and enumeration of lipolytic microogranisms.
Components
Ingredients (g/L)
Casein enzymic hydrolysate, 10.00
Yeast extract, 5.00
Spirit blue, 0.15
Agar, 17.00
Casein enzymic hydrolysate, 10.00
Yeast extract, 5.00
Spirit blue, 0.15
Agar, 17.00
Preparation Note
Suspend 32.15 g of Spirit Blue Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and add slowly 30 ml of the lipase substrate while agitating to obtain an even distribution.
Note: To prepare the lipase substrate mix 1 ml of Tween 80 (Cat. No. 93780) in 400 ml warm distilled water. Add 100 ml of Cottonseed Oil (Cat. No. C7767) or Olive Oil (Cat. No. 75343). Agitate vigorously to emulsify.
Note: To prepare the lipase substrate mix 1 ml of Tween 80 (Cat. No. 93780) in 400 ml warm distilled water. Add 100 ml of Cottonseed Oil (Cat. No. C7767) or Olive Oil (Cat. No. 75343). Agitate vigorously to emulsify.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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