Skip to Content
Merck
All Photos(3)

Key Documents

PPB013

Sigma-Aldrich

1.0% Agarose in Tris-Borate EDTA Buffer

pHast Pack, powder

Synonym(s):

1% Agarose gel, TAE Agarose blend, TBE Agarose blend, TBE buffer garose blend

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105317
NACRES:
NA.25

product line

pHast Pack

Quality Level

form

powder

pH

8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose)

cation traces

Pb: ≤5 ppm

foreign activity

DNase, none detected
Endonuclease
Exonuclease, none detected
NICKase, none detected
RNase, none detected

General description

1% Agarose in Tris Borate EDTA (TBE buffer, pH 8.3) is a powder blend that dissolves easily in boiling water to quickly make an agarose gel for electrophoresis. Typically, 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp

Application

1% Agarose gel in 1X TBE buffer may be used for:
  • DNA electrophoresis
  • Native RNA Electrophoresis

Features and Benefits

  • Ready-to-pour 1% agarose gel eliminates buffer prep
  • Accelerates workflow and minimize cost and labor
  • Biological tests: free of DNase, RNase, Protease, and Nickase
  • Chemical tests: Iron ≤10 ppm Lead ≤5 ppm
  • Compatible with TBE pHast pack buffer

Packaging

Foil pouches

Preparation Note

Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3.

Reconstitution

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Storage and Stability

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service