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A5144

Sigma-Aldrich

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate

≥95% purity (perchloric acid titration), for fluorescence, powder

Synonym(s):

1,8-ANS magnesium salt, ANSA magnesium salt, Magnesium 8-anilino-1-naphthalenesulfonate hydrate, N-Phenyl peri acid magnesium salt

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About This Item

Empirical Formula (Hill Notation):
C16H12Mg0.5NO3S · xH2O
CAS Number:
18108-68-4
Molecular Weight:
310.49 (anhydrous basis)
Beilstein:
4834623
EC Number:
242-004-7
MDL number:
MFCD16661224
UNSPSC Code:
12171500
PubChem Substance ID:
329770870
NACRES:
NA.47

product name

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate, for fluorescence, ≥95% (TLC)

grade

for fluorescence

Quality Level

200

Assay

≥95% (TLC)

form

powder

color

faint green to green

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

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General description

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL1990
BCCC9821
BCCK8670
BCCK2567
BCBR1899V

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Silvia Zorrilla et al.
Methods in molecular biology (Clifton, N.J.), 952, 237-252 (2012-10-27)
Transcription factors of the peroxisome proliferator-activated receptor (PPAR) family are ligand-activated receptors that play key roles in lipid metabolism and inflammation. The γ isoform (PPARγ) is involved in adipocyte differentiation, insulin sensitization, and vascular pathophysiology, including inflammation and atherosclerosis, for
Shilpi Gupta et al.
Small (Weinheim an der Bergstrasse, Germany), 9(6), 894-904 (2012-12-12)
A new class of non-ionic dendronized multiamphiphilic polymers is prepared from a biodegradable (AB)n-type diblock polymer synthesized from 2-azido-1,3-propanediol (azido glycerol) and polyethylene glycol (PEG)-600 diethylester using Novozym-435 (Candida antarctica lipase) as a biocatalyst, following a well-established biocatalytic route. These
Tsuyoshi Konuma et al.
Proteins, 81(1), 107-118 (2012-08-29)
Chemical shift perturbations (CSPs) in NMR spectra provide useful information about the interaction of a protein with its ligands. However, in a multiple-ligand-binding system, determining quantitative parameters such as a dissociation constant (K(d) ) is difficult. Here, we used a
Zhenyu Zuo et al.
The protein journal, 31(7), 585-591 (2012-08-10)
The interactions between Urinary Trypsin Inhibitor (UTI) and 1-anilino-8-naphthalene sulfonate (ANS) were investigated by fluorescence spectra, isothermal titration calorimetry and molecular modeling. The results revealed the presence of four specific binding sites for ANS on UTI, with interactions driven mainly
Gulam Rabbani et al.
Cell biochemistry and biophysics, 62(3), 487-499 (2012-01-05)
Here, we have characterized four pH-dependent states: alkaline state, "B" (pH 9.0), native state, "N" (pH 7.4), acid-induced state, "A" (pH 2.2) and molten globule state, "MG" (pH 1.8) of Rhizopus niveus lipase (RNL) by CD, tryptophanyl fluorescence, ANS binding
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