2GRHSNTKB
Roche
KAPA2G Robust HotStart PCR Kit
with dNTPs
Synonym(s):
PCR, hotstart PCR
About This Item
Recommended Products
Quality Level
usage
sufficient for 1000 reactions
sufficient for 200 reactions
sufficient for 500 reactions
shelf life
≤12 mo.
feature
Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart
packaging
pkg of 100 U (KK5532)
pkg of 250 U (KK5516)
pkg of 500 U (KK5518)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
crude DNA
storage temp.
−20°C
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General description
Application
- Amplification of GC- or AT-rich templates
- Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
- Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
- Colony PCR
- qPCR
- Nested polymerase chain reaction (PCR) amplification
- Amplification of DNA targets in PCR
- DNA amplification using conventional PCR
- Routine PCR
Biochem/physiol Actions
Features and Benefits
- Robust performance across a wide range of GC- and AT-rich templates
- Increased PCR success rates
Improved tolerance to common PCR inhibitors
- Efficient amplification from crude samples
- Higher yield and sensitivity per unit of enzyme
Unrivalled performance in colony PCR
- Higher yields and improved consistency of PCR direct from E. coli and yeast cells
Quick Notes:
- KAPA2G Robust HotStart® PCR Kits with dNTPs contain KAPA2G Robust HotStart DNA Polymerase, engineered for high processivity and inhibitor tolerance.
- Both purified genomic DNA and crude samples (e.g. colony PCR) can be used as template.
- Use 0.5 U of KAPA2G Robust HotStart DNA Polymerase per 25 μL reaction. More challenging PCRs (GCrich,crude sample) may require higher enzyme concentrations.
- Use 15 sec/kb extension time per cycle, and increase to 30-60 sec/kb for difficult amplicons or templates.
- KAPA2G Buffers contain 1.5 mM MgCl2 at 1X.
- Additional MgCl2 may be added using the 25 mM MgCl2 solution provided in the kit.
- KAPA2G Buffer A is optimized for high yield, specificity, and sensitivity.
- KAPA2G Buffer B is recommended for inhibitorcontaminated template samples.
- KAPA2G GC Buffer is recommended for GC-rich PCR (>70% GC).
- KAPA Enhancer 1 can be used with KAPA2G Buffer A or B, particularly for GC-rich assays.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Legal Information
Kit Components Only
- KAPA2G Robust HotStart® DNA Polymerase 5 U/µL
- 5X KAPA2G Buffer A
- 5X KAPA2G Buffer B
- 5X KAPA2G GC Buffer
- 5X KAPA Enhancer 1
- KAPA MgCl2 25 mM
- KAPA dNTP Mix 10 mM each
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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