2GFNTKB
Roche
KAPA2G Fast PCR Kit
with dNTPs
Synonym(s):
PCR, PCR kit
About This Item
Recommended Products
Quality Level
usage
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Fast PCR
Multiplex PCR
dNTPs included
hotstart: no
packaging
pkg of 100 U (KK5008)
pkg of 250 U (KK5009)
manufacturer/tradename
Roche
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
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General description
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
Application
- PCR with confronting 2 -pair primers assay (PCR-CTPP)
- Routine PCR
- Fast PCR
- Genotyping
Biochem/physiol Actions
Features and Benefits
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast PCR Kits with dNTPs contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
- Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Kit Components Only
- KAPA2G Fast Standard or HotStart® DNA Polymerase (5 U/μL)
- 5X KAPA2G Buffer A with MgCl2
- MgCl2 25 mM
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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