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2GFNTKB

Roche

KAPA2G Fast PCR Kit

with dNTPs

Synonym(s):

PCR, PCR kit

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.54

Quality Level

usage

sufficient for 500 reactions

shelf life

≤18 mo.

feature

Fast PCR
Multiplex PCR
dNTPs included
hotstart: no

packaging

pkg of 100 U (KK5008)
pkg of 250 U (KK5009)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

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General description

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

Application

KAPA2G Fast PCR Kit has been used in:
  • PCR with confronting 2 -pair primers assay (PCR-CTPP)
  • Routine PCR
  • Fast PCR
  • Genotyping

Biochem/physiol Actions

KAPA2G Fast DNA Polymerase generated DNA fragments is useful in sequencing restriction enzyme digestion and cloning experiments. KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities like wild-type Taq, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.

Features and Benefits

Reduce cycling times up to 75% :
  • Extension times as low as 1 sec per kilobase
  • Broad coverage of both AT- and GC-rich targets

High speed, higher performance :
  • Single copy sensitivity from complex targets
  • Increased yields

Quick Notes :
  • KAPA2G Fast PCR Kits with dNTPs contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
  • Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
  • No need for specialized instrumentation or PCR consumables.
  • Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
  • Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
  • For high reaction efficiency, do not exceed 25 μL reaction volumes.
  • KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.
  • Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

Quality

Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • KAPA2G Fast Standard or HotStart® DNA Polymerase (5 U/μL)

  • 5X KAPA2G Buffer A with MgCl2

  • MgCl2 25 mM

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kazuyuki Hoshijima et al.
Developmental cell, 51(5), 645-657 (2019-11-12)
Inconsistent activity limits the use of CRISPR-Cas9 in zebrafish. We show supernumerary guanine nucleotides at the 5' ends of single guide RNAs (sgRNAs) account for diminished CRISPR-Cas9 activity in zebrafish embryos. Genomic sequences can be targeted consistently with extremely high
Nevin M Al Azhary et al.
Asian Pacific journal of cancer prevention : APJCP, 17(11), 4945-4949 (2016-12-30)
Background: Breast cancer is the commonest cancer in Egyptian females. Nrf2 is involved in oxidative stress while P73 functions in response to DNA damage. This study aimed to assess the role of Nrf2 promoter and P73 G4C14 to A4T14 SNPs
Elisa Bergamo et al.
Journal of virological methods, 181(1), 125-130 (2012-01-24)
The potential advantage of using fast PCR to detect human herpesvirus 8 (HHV-8) was tested by running a rapid cycling protocol (5s-steps) in one standard and two fast ramping thermal cyclers to evaluate the performance of 8 different fast reagents.
Klotho G395A Polymorphism in Egyptian Patients with Diabetic Nephropathy and Hemodialysis
Nasr HG Abd El-Aziz, et al.,
Al-Azhar Medical Journal, 331, 1-13 (2013)

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