MABF297
Anti-RIG-I, clone 1C3 Antibody
clone 1C3, from mouse
Synonym(s):
Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1C3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1λ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DDX58(23586)
General description
Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants.
Immunogen
Recombinant protein corresponding to human RIG-I.
Application
Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-549 infected with SeV cell lysate (Baum, A., et al. (2011). PNAS. 108(7):16303-16308).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-459 lung carcinoma cell lysate (Nistal-Villan, E., et al. (2010). J. Biol. Chem. 285:20252-20261).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RIG-I in HeLa cells (Courtesy of Maite Sanchez).
Immunoprecipitation Analysis: A representative lot immunoprecipitated RIG-1 in A-459 lung carcinoma cell lysate (Nistal-Villan, E., et al. (2010). J. Biol. Chem. 285:20252-20261).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected RIG-I in HeLa cells (Courtesy of Maite Sanchez).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-RIG-I, clone 1C3 Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of RIG-I.
Quality
Evaluated by Western Blotting in A-549 infected with SeV cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RIG-1 in 10 µg of A-549 infected with SeV cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected RIG-1 in 10 µg of A-549 infected with SeV cell lysate.
Target description
~110 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Philippa Hillyer et al.
Journal of virology, 92(15) (2018-05-18)
Respiratory syncytial virus (RSV) infects small foci of respiratory epithelial cells via infected droplets. Infection induces expression of type I and III interferons (IFNs) and proinflammatory cytokines, the balance of which may restrict viral replication and affect disease severity. We
Jie Chen et al.
Cell reports, 32(9), 108080-108080 (2020-09-03)
The DNA-dependent pattern recognition receptor, cGAS (cyclic GMP-AMP synthase), mediates communication between the DNA damage and the immune responses. Mitotic chromosome missegregation stimulates cGAS activity; however, it is unclear whether progression through mitosis is required for cancercell-intrinsic activation of anti-tumor
Na Li et al.
Cell reports, 35(6), 109091-109091 (2021-05-08)
It is urgent and important to understand the relationship of the widespread severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) with host immune response and study the underlining molecular mechanism. N6-methylation of adenosine (m6A) in RNA regulates many physiological and
Daniel T Thoresen et al.
Molecular cell, 83(1), 90-104 (2022-12-16)
RIG-I is essential for host defense against viral pathogens, as it triggers the release of type I interferons upon encounter with viral RNA molecules. In this study, we show that RIG-I is rapidly and efficiently activated by small quantities of
Nicholas R Meyerson et al.
Cell host & microbe, 22(5), 627-638 (2017-11-07)
TRIM25 is an E3 ubiquitin ligase that activates RIG-I to promote the antiviral interferon response. The NS1 protein from all strains of influenza A virus binds TRIM25, although not all virus strains block the interferon response, suggesting alternative mechanisms for
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