6502

Trypsin, Bovine Pancreas

• Synonym(s): Trypsin, Bovine Pancreas
• CAS Number: 9002-07-7
• EC Number: 3.4.21.4 (BRENDA, IUBMB)
• NACRES: NA.25
• UNSPSC Code: 12352202
• MDL number: MFCD00040513

Properties

• description: RTECS - YN5075000; Merck USA index - 14, 9795
• Quality Segment: 100
• form: solid
• specific activity: ≥2,500 USP units/mg dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• solubility: HCl: 1 mM, neutral buffer: soluble, water: soluble
• foreign activity: Chymotrypsin ≤50 NF units/mg dry wt.
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.

Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.

Physical form

Salt-free

Preparation Note

Following reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.

Other Notes

Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3^rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem.239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol.37, 1393.

One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Codes: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• WGK: WGK 1