345860

Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)

liquid, clone 2.2B10, Calbiochem^®

• Synonym(s): Anti-GFAP
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2.2B10, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: bovine, rat, mouse, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GCM1(8521)

Description

General description

Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).

Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.

This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

Immunogen

Bovine Brain

bovine GFAP

Application

ELISA (0.1-0.5 µg/ml)

Immunoblotting (0.1-0.5 µg/ml)

Frozen Sections (10-50 µg/ml)

Immunoprecipitation (2-5 µg/ml)

Paraffin Sections (10-50 µg/ml, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human or rat brain tissues

Other Notes

Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.

Tohyama, T., et al. 1993. Am. J. Pathol.142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable