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Merck

14-418

Sigma-Aldrich

p300, HAT Domain

Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

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biological source

human

Quality Level

100

recombinant

expressed in E. coli

mol wt

Mw 100 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (histone acetyltransferase (HAT))

NCBI accession no.

NM_001429.2

UniProt accession no.

Q09472

shipped in

dry ice

Gene Information

human ... EP300(2033)

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This Item
14-49414-595-M14-309
p300, HAT Domain Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.

14-418

p300, HAT Domain

Histone H3, human recombinant Recombinant human histone H3.

14-494

Histone H3, human recombinant

PP1α Protein, 10 µg Recombinant, full length, human PP1α, for use in Phosphatase Assays.

14-595-M

PP1α Protein, 10 µg

PCAF Protein, Active, 50 µg PCAF recombinant GST fusion protein, for use in Histone Acetyltransferase Assays. (HAT).

14-309

PCAF Protein, Active, 50 µg

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

activity assay: suitable (histone acetyltransferase (HAT))

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable (phosphatase)

technique(s)

activity assay: suitable (histone acetyltransferase (HAT))

biological source

human

biological source

human

biological source

human

biological source

-

mol wt

Mw 100 kDa

mol wt

Mw ~17 kDa

mol wt

Mw 37.6 kDa

mol wt

Mw 79 kDa

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

dry ice

Gene Information

human ... EP300(2033)

Gene Information

human ... H3F3B(3021)

Gene Information

human ... PPP1CA(5499)

Gene Information

-

General description

Product Source: Expressed in E. coli.

Application

Recombinant GST-fusion protein corresponding to amino acids 1066-1707 of human p300.

Preparation Note

-20°C for up to 6 months from the date of receipt.

Analysis Note

Routinely evaluated by HAT Assay.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme
Specific Activity: 1 Unit is defined as the transfer of 1nmol of [3H]-acetate/minute to acid-extracted histones.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The acetylation of transcription factor HBP1 by p300/CBP enhances p16INK4A expression.
Weibin Wang,Kewu Pan,Yifan Chen,Chunyin Huang,Xiaowei Zhang
Nucleic Acids Research null
A Giordano et al.
Journal of cellular physiology, 181(2), 218-230 (1999-09-25)
p300 and CBP are highly related nuclear proteins, which have been implicated in transcriptional responses to disparate extracellular and intracellular signals. There are at least two very good reasons for which p300 and CBP have attracted the attention of the
R L Schiltz et al.
The Journal of biological chemistry, 274(3), 1189-1192 (1999-01-09)
A number of transcriptional coactivators possess intrinsic histone acetylase activity, providing a direct link between hyperacetylated chromatin and transcriptional activation. We have determined the core histone residues acetylated in vitro by recombinant p300 and PCAF within mononucleosomes. p300 specifically acetylates
Dennis Shin-Shian Hsu et al.
Cancer cell, 26(4), 534-548 (2014-10-15)
Snail is primarily known as a transcriptional repressor that induces epithelial-mesenchymal transition by suppressing adherent proteins. Emerging evidence suggests that Snail can act as an activator; however, the mechanism and biological significance are unclear. Here, we found that CREB-binding protein
Junhui Xue et al.
Cancer letters, 471, 12-26 (2019-12-08)
Alpha-fetoprotein (AFP) is a well-established biomarker for hepatocellular carcinoma (HCC). Here, we investigated the acetylation state of AFP in vivo. AFP acetylation was regulated by the acetyltransferase CBP and the deacetylase SIRT1. Acetylation of AFP at lysines 194, 211, and

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