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Key Documents

126579

Sigma-Aldrich

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

Synonym(s):

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free

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About This Item

CAS Number:
9048-46-8
UNSPSC Code:
12352202
NACRES:
NA.25

Assay

≥95% (Albumin, electrophoresis)

form

lyophilized powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.2% fatty acids
≤2.0% Ash
≤5% moisture
≤50 EU/mg endotoxins

color

pale green

solubility

aqueous buffer: 20 mg/mL
water: 20 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free is designed for use in cell culture systems and animal perfusion studies. It has a pH of 7.0 (1% solution).
Bovine serum albumin (BSA), a member of the serum albumin family, contains three domains and two sub-domains under each. It is a non-glycosylated globular, α-helical protein that has 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, Fatty Acid-Poor, Endotoxin-Free has been used in the preparation of advanced glycation end-products (AGEs). It has also been used to dilute lipopolysaccharide (LPS) and tumor necrosis factor (TNF) and in the stimulation and fixation of whole blood cells.

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most important constituent of cell culture media. It aids embryonic stem cells (hESC) differentiation. BSA facilitates the transport of drugs, hormones, and fatty acids. It elicits high solubility and is suitable in the cosmetic and pharmaceutical industries. It is applicable as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Warning

Toxicity: Standard Handling (A)

Preparation Note

Material may take up to 24 h to fully dissolve.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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