Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

1.00432

Millipore

ChromoCult® Listeria Agar Selective-Supplement

for Listeria spp., pkg of 10 vials, for the preparation of ChromoCult® Listeria agar (base)

Synonym(s):

Listeria Selective Supplement

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

Agency

ISO 11290

Quality Level

100

description

ChromoCult™ Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)

form

lyophilized powder

composition

Amphotericin B, 5 mg per vial
Ceftazidime, 10 mg per vial
Nalidixic acid sodium salt, 10 mg per vial
Polymyxin B sulphate, 38350 (U per vial)

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

pH

6.2 (20 °C in H2O, Aqueous solution)

application(s)

food and beverages
microbiology

storage temp.

2-8°C

suitability

Listeria spp.

General description

ChromoCult® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.

Application

FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult® Listeria agar (base) for the selective growth of Listeria spp.

Linkage

Replaces: 1.00432.0017

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334,H351,H373

Hazard Classifications

Carc. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service